《植物生理学报》 2018, 54(9): 1459-1466
通信作者:徐飞;E-mail: feixu666@hotmail.com, zhq_he@163.com
摘 要:
我们前期研究发现拟南芥AT2G15020基因受氰化氢(HCN)显著诱导, 该基因编码一个未知功能蛋白。为探究该基因的功能, 本研究对AT2G15020基因进行了生物信息学分析, 包括同源序列比、启动子分析、蛋白理化性质及蛋白互作预测等。生物信息学分析结果表明, 与该基因同源蛋白均为未知功能蛋白, 但该基因启动子中存在较多光响应元件, 与其互作蛋白也显示其与光响应或光信号调节有关。不同光照强度和盐胁迫研究结果表明, AT2G15020基因响应光强变化和盐胁迫处理, 维持该基因较高表达水平有助于增强植株在逆境条件下的光合效率。关键词:生物信息学分析; 氰化氢; 光响应; 胁迫适应
收稿:2018-04-04 修定:2018-07-25
资助:国家自然科学基金(31400242、31271686)和湖北省优秀中青年科技创新团队项目(T201732)。
Corresponding author: XU Fei; E-mail: feixu666@hotmail.com, zhq_he@163.com
Abstract:
Although the genome sequencing of Arabidopsis thaliana has already been uncovered for a long time, a large number of gene functions are still unknown. Our previous study found that the AT2G15020 gene was significantly induced by hydrogen cyanide (HCN), but it is an uncharacterized protein. In order to explore the function of AT2G15020 gene, the bioinformatics analysis was performed in this study, including protein sequences align, promoter analysis, physiological and biochemical characters and protein interactions prediction. The results of bioinformatics analysis shows that the homologous proteins of AT2G15020 are all uncharacterized proteins. However, promoter analysis shows that there are a lot of cis-acting elements in the promoter of the AT2G15020 gene, and the protein interaction analysis of AT2G15020 gene further indicates that it is possible related to light response or light signal regulation in plants. Additionally, it should be noted that the AT2G15020 gene is sensitive to light intensity and salt stress, and the higher expression level of AT2G15020 gene contributes to plant photosynthetic efficiency under adverse condition.Key words: bioinformatics analysis; hydrogen cyanide; light response; stress tolerance
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